Annotation: The article is devoted to comprehensive research and development of the technology of cream supplements for the enrichment of processed cheese products. This article discusses the steady trend towards the use of dairy products with probiotic properties in the diet of modern people. The authors justify the requirements for the chemical composition and properties of a new type of processed cheese product: the mass fraction of dry processed cheese product must be at least 45 wt.%, the amount of probiotic microflora at least 107 CFU/g, to adjust the fatty acid composition of the product, the use of milk fat substitutes. As a result of the research, the composition and technological parameters of the production of a cream Supplement intended for enriching a new processed cheese product were determined. Skimmed milk powder concentrate with a mass fraction of dry substances (48.0±0.5) %was selected to increase protein (nitrogenous substances) in the nutrient medium for microorganisms. To activate the growth of bifidobacteria, the prebiotic No. 1 – lactulose was studied. The main content of the study is the analysis of the effect of increasing the amount of SOM concentrate on the chemical composition of the compositions of experimental products. The bacterial concentrates Bifilact-B and Bifilact-u were selected as the source of probiotic cultures. In fermented products, microbiological indicators were determined: the total number of lactic acid cultures and bifidobacteria. The article analyzes the dynamics of acidity of experimental products fermented with bifilact-B bacterial concentrate and bifilact-u bacterial concentrate. It was found that in experimental products with a high level of acid formation, the growth of bifidobacteria decreases, this is due to the fact that at low pH values, the growth of bifidobacteria slows down, and at a pH below 4.5 – stops. There was a significant increase in the number of bifidobacteria in the presence of a prebiotic – lactulose concentrate. The authors proved that the fermentation process with the formation of a clot in experimental products with bifilact-B bacterial concentrate is completed within 9-10 hours, taking into account the time of compaction of the consistency. In experimental products fermented with bifilact-U bacterial concentrate, the fermentation process ends within 7-8 hours, that is, by (2.0±0.5) hours faster.

Annotation: Main problem: Microbial-plant relationships, including epiphytic microflora, are the subject of attention of many scientists. Numerous works confirm the high interest of researchers and the relevance of studying this topic. Despite many years of research, some questions concerning the characteristics of the epiphytic microflora still remain open. It is already a well-known fact that the epiphytic microflora is directly related to the physiological development of the plant, including yield. This is due to the close interaction of the plant with the microorganisms living on its surface throughout the entire growing season. It is also proved that epiphytic microflora has the ability to change characteristics under the influence of environmental factors (temperature, humidity, soil contamination, sunlight, etc.). In this regard, the study of this topic is one of the important areas of biological and agricultural sciences and is of high importance. It should be noted that a significant role in the development of agriculture in the Republic of Kazakhstan and the Pavlodar region is played by representatives of vegetable crops, including tomatoes and potatoes belonging to the Solanaceae family, which indicates the high importance of the plants selected for the study. The combination of the above arguments determines the need to study the epiphytic microflora of plants and establish the regularities of its influence on the adaptive properties of the studied plants of the Solanaceae family, as well as on their productivity. In turn, the study of epiphytic microflora can also contribute to the search for new ways to increase the yield of plants and their resistance to various diseases. Purpose: The article is devoted to the study of the properties and characteristics of the epiphytic microflora of the surface of various organs of plants of the Solanaceae family (on the example of tomato and potato). The variability of the composition and number of epiphytic microorganisms in seasonal dynamics is shown. Methods: bacterioscopic method, fingerprint method, flushing method, Gram staining method. Results and their significance: The characteristics and properties of representatives of epiphytic microorganisms of aboveground (leaf, fruit, flower) and underground (potato fruit) plant organs were studied. The role of the influence of environmental factors on the variability of epiphytic microflora is determined on the example of plants Lycopersicon esculentum Mill. (common tomato) and Solanum tuberosum L. (tuberous nightshade). The differences in the microflora of different plant organs in different periods of vegetation are shown.

Annotation: Main problem: The development of the genetic potential of poultry, as well as the receipt of high-quality eggs for collection or further incubation, are possible only if rational and correct feeding of the broodstock and, more importantly, the young during its rearing period is observed. During this period, high-quality protein nutrition occupies an important place, which determines the level of productivity, stable growth, reproductive ability of the bird, as well as the state of health during the feeding period. Protein deficiency always remains an urgent problem for agricultural land, whose main activity is aimed at breeding dairy and meat animals. In this regard, research in the field of finding the use of non-traditional protein feed is of great need. If we summarize the modern achievements in the field of protein and aminoacid nutrition of poultry, we can find the most profitable way to obtain feed additives in industrial production. The studies, the results of which will be described in this article, are aimed at studying the possibility, experimental substantiation of the optimal dosage and the effectiveness of using the feed additive from crustaceans Artemia salina in the diets of chickens of the egg-meat direction of productivity [1]. Purpose: To study the possibility, experimental substantiation of the optimal dosage and the effectiveness of the use of the feed additive from crustaceans Artemia salina in the diet of chickens of the egg-meat direction of productivity. Methods: Analysis and generalization of theoretical information Results and their significance: This article presents the results of the analysis and generalization of theoretical material. The theoretical significance is determined by the fact that it is invested in scientific and practical problems related directly to solving urgent problems, increasing the efficiency, competitiveness and quality of domestic poultry products.

Annotation: The development of extraction techniques of medical plants aims to maximize the bioactive compounds production. Тechnologies in this area of industry have not high enough еfficiency factor. In some cases, it reaches only 40-50 %. Therefore, development of new methods to effectively extract different compounds from plant material and their input into large-scale industry is of great importance. The goal is to determine an effective and suitable technology for extracting bioactive compounds from medical plant. This is a narrative review paper which focuses on different extraction methods of medical plant. To find relevant articles for this narrative review, 73 articles were reviewed. Of these, 14 irrelevant documents were discarded. This review analyses various extraction processes to guide the selection of suitable methods for various types of medical plants and applications. This is done by outlining traditional and modern methods of extraction techniques, exploring the importance of solvents for extraction, and comparing novel and alternative methods of extraction. In conclusion, based on the current knowledge, solvent extraction is the most common method for medical plants extraction. Enzymatic hydrolysis and supercritical extraction are advanced techniques with high yield of compounds. However, more research is needed for some modern extraction methods.

Annotation: Main problem: The unfavorable conditions in which a significant part of the population of our country lives (living in conditions of harmful, stressful, physical, chemical and radiation effects), an imbalance in nutrition dictate the need to create a variety of functional products enriched with pro– and prebiotic factors. In the science of nutrition, a functional direction has been developed - this is nutrition, which provides for the consumption of such products of natural origin, which, when used daily, have a regulating effect on the body as a whole, its certain systems, organs, providing a drug-free positive correction of their function. Compared with traditional types of dairy products with high organoleptic and commodity characteristics, produced on the basis of classical technological techniques, dairy products of the new generation should have increased nutritional and biological value. The article is devoted to the research and development of the technology of a new cottage cheese product - cottage cheese paste produced with the addition of lactulose concentrate and fruit and berry fillers. This article discusses the steady trend towards the use of fermented milk products with functional properties in the diet of modern humans. Purpose: Study and development of technology of thermized cottage cheese paste enriched with prebiotic lactulose and fruit and berry fillers. Methods: Analysis and generalization of theoretical information, organoleptic evaluation, physicochemical methods of analysis, microbiological methods of evaluation of cottage cheese paste, quality control of the finished product. Results and their significance: The positive role of prebiotic substances in the vital activity of the macroorganism was studied. It is not for nothing that academician Andrei Georgievich Khramtsov once called lactulose "a miracle made of milk." Lactulose, being a strong bifidogenic factor, allows stabilizing the microflora of the stomach. Lactulose activates the growth of bifidobacteria, which take root well in the intestine and ferment lactulose to form lactic acid. Lactic acid suppresses the development of pathogenic and putrefactive bacteria. As a result of the conducted studies, it was determined that the cottage cheese paste enriched with lactulose had high quality indicators and retained its consumer properties during the entire storage period. The introduction of lactulose into the recipe of cottage cheese paste does not reduce the nutritional advantages of the finished product.

Annotation: The variety of species and forms of parasites of agricultural animals and the features of their life cycles are the basic foundation for the production of different types and medical forms of antiparasite medicine. In the treatment of the parasitic diseases of agricultural animals, one of the main factors is that the veterinary doctors have the knowledge on the mechanics, efficiency and the safety for humans and environment of the medicine being used. Taking into account high pathogenicity and resistance of parasitic organisms and their ability to adapt to effect of medicine, it is crucial to permanently monitor the efficiency of the applied anthelmintic drugs, including the new drugs offered by veterinary services. This article will describe the results of the research on pharmacodynamic and pharmacokinetic parameters of «Ivermectinum», «Ivermec», «Aversect-1», «Аvrsect-2», «Аivin» drugs and their methods of application with the consideration of terms of maintenance and immune status of cattle herds in Mayconyr village (Irtysh district, Pavlodar region). A comparative analysis of efficiency of the above mentioned drugs in dehelmintization and treatment of cattle was conducted with the results provided. The recommendations and the restrictions on use of the drugs were determined considering the farm condition. The experimental research of the aforementioned broad-spectrum drugs was conducted strictly following the rules of asepsis and instructions on application. The total number of replacement heifers subjected to the dehelmintization under the method of analogues is 150 units. Anthelmintic treatment was conducted two times a year - in springtime (March-April) before pasturage, and fall time (September-October) before confining the cattle in the stable. Coprological study on intestinal helminthes of the examined cattle was conducted in Irtysh RVL of the Pavlodar regional branch of RVE RVL VCaSC MA RK by ovoscopical, flotation and larvoscopical methods. There is a comparative analysis on the efficiency of avermectin group antiparasitic drugs applied twice a year for cattle dehelmintization. The recommendations on the use of the aforementioned drugs for dehelmentization and prevention of parasitic diseases are provided and the requirements for the use were determined.

Annotation: Main problem: Bird droppings and animal manure contain acids, nitrogen, phosphorus and potassium, heavy metals. The content of nitrogen, phosphorus and potassium changes dramatically depending on the quantity and quality of the feed. Purpose: To study the effectiveness of the use of organomineral fertilizer from poultry waste, obtained using biocatalytic processes on the growth and development of plants. Methods: An application for a Patent for the invention of the Republic of Kazakhstan "Method of obtaining organic fertilizer" has been submitted for the developed technology for obtaining organic-mineral fertilizer. In the soil intended for growing seedlings of tomatoes of the “Pepper-shaped Orange” variety and peppers of the “Bogatyr” variety, the resulting fertilizer was applied in the amount of 1 kg per 1 m2 of soil, which was dug to a depth of 8-10 cm and used to grow seedlings in closed ground and subsequent for planting it in open ground. Soil without fertilization was used as a control. The process of soil preparation for open ground, intended for planting seedlings, was carried out for the experimental field with the introduction and control without fertilization. Experimental and control studies were carried out under equivalent climatic conditions, the scheme and technology of watering plants. In total, 100 bushes of each plant species were used in the experiment. Results and their significance: The use of the obtained organomineral fertilizer allows to increase the yield of vegetable crops (tomatoes, peppers) by 20-25% compared to the control. In addition, in the experimental samples, an increase in the number of fruits on a bush was noted with an increase in their size, a decrease in the growing season and the number of damaged fruits.

Annotation: In this article, comprehensive studies have been conducted on the effective use of natural pastures in conditions of vertical zonality in the project territory of Batyr Korday district (Zhambyl region). The data presented shows how important it is to comply with the norms and loads of pastures for further preservation of pasture yields and prevention of degradation. Consequently, there is a need to develop effective and scientifically-based systems of pasture turnover and the introduction of measures to restore pasture lands in the Zhambyl region, taking into account the peculiarities of the climate and geo-botanical indicators. Pasture lands make up about seventy percent of the entire territory of the Republic of Kazakhstan. The potential productivity of these lands reaches twenty-five and more million tons of fodder units. Pasture is the main renewable forage plant resource. It should be emphasized that haphazard use has led to the fact that land degradation processes occur on 27.5 million hectares of pastures, feed capacity decreases, biological diversity decreases, erosion processes manifest themselves, etc. All this requires the development of science-based approaches to the exploitation of pasture resources, compliance with the basic elements of rational grazing. The current situation may have a twofold path of development. The first way is the absence of strategic initiatives for the effective use of pastures for small producers will lead to a final reduction in the feed capacity of the pastures used. And the second way is small and large-scale use of the huge forage potential of the restored pastures.

Annotation: The production of vaccine preparations before release requires standardization of their immunobiological parameters, especially safety and immunogenic efficacy. An indicator of the immunogenic effectiveness of the lumpy skin disease vaccine is the resistance of vaccinated cattle against the virulent virus. However, according to preliminary studies, the virulent control virus did not always cause clinical disease with characteristic symptoms when infected subcutaneously. The purpose is to develop a biological model in the form of a complex consisting of a pathogenic virus, a method of infection and a susceptible animal to assess the immunogenicity of a lumpy skin disease vaccine. Local cattle, intact from lumpy dermatitis, were used to reproduce lumpy dermatitis and develop the causative agent of the disease. As the initial infectious virus, we used a 20 % tissue suspension of nodules (skin nodules) obtained from cattle that fell ill with lumpy dermatitis in the field in the Atyrau region in 2016. As a viral mass to control immunogenicity, a 20 % suspension of skin nodules and edematous skin tissue at the site of the pathogen injection, obtained after the “refreshment” of the virus in animals, were used. The disease was reproduced by infection with the test suspension of the virus intradermally, subcutaneously, intravenously at a dose of 0.5 cm3 and titration on the skin of the animal. The effectiveness of the biological model was assessed by morbidity, severity of the course and severity of the manifestation of the disease. During primary intradermal infection with a field isolate of the virus, the disease manifested itself in one of three animals in the form of hyperthermia, depression, lacrimation, and the appearance of several nodular nodules in the skin of animals. The refreshed tissue virus caused clinical disease both in subcutaneous, intradermal and intravenous infection. But the clinical signs of the disease were more pronounced with intradermal inoculation of the virus, and with intravenous inoculation, it manifested itself in a more severe form with a fatal outcome. Inoculation of the virus intradermally into different areas of the skin led to the development of an independent skin lesion in each infected point in the form of painful edema, followed by necrosis, the size and intensity of which depended on the dose of the injected virus. This development of skin pathology made it possible to work out a method for determining the virus titer in vivo. The tissue virus obtained from the edematous tissue at the site of the pathogen injection was guaranteed to cause clinical disease in cattle during intradermal inoculation and made it possible to evaluate the immunogenic efficacy of the produced batches of vaccine against lumpy dermatitis.

Annotation: The degree of dаmаge to deer by the lаrvаe of Oedemаgenа tаrаndi of the Hypodermаtidаe fаmily depends on the number of femаles of the subcutаneous gаdfly in the summer. For the development of meаsures to combаt deer gаdflies, vаrious methods hаve been tested over the yeаrs, аmong them methods of wаtering аnd externаl locаl аpplicаtion. For this purpose, mаny reseаrchers tested dipterex (80 %), crystаlline chlorophos (97 %) in the form of аn 8 % solution, tiguvon, ricifon, dioxаfos, sulfidophos-20 аnd ectopor (2 % cypermethrin) аnd other drugs. Vаrbeks 35 %, Bаytex-50, Fenthion-50, Nаtаsol аnd Etаcid, etc. were tested intrаmusculаrly in vаrious doses on deer. The purpose is to study the prevаlence аnd effectiveness of the treаtment of edemаgenosis in sikа deer in the Ussuriysk urbаn district. The reseаrch wаs cаrried out аt the deer fаrm "Borisovskаyа". For the experiment, three groups of deer were formed with 6 heаds eаch. In order to study the therаpeutic аnd prophylаctic efficаcy of the two groups of drugs Аversect-2 аnd Dectomаx, the аnimаls of the first experimentаl group were injected with the drug Аversect-2 subcutаneously аt the rаte of 1 ml per 50 kg of аnimаl weight, deer of the second experimentаl group were injected subcutаneously with Dectomаx аt а dose of 0.2 mg per 1 kg of mаss, which corresponds to 1 ml of solution per 50 kg of mаss. The prepаrаtions were аdministered immediаtely аfter the end of the summer of gаdflies (end of July-beginning of Аugust). The third group of deer did not receive drugs, аnd it remаined the control group. The incidence of sikа deer entomosis in the Ussuriysk urbаn district for three yeаrs wаs quite high аnd remаined аt the sаme level for аlmost the entire period (43.21-48.8), with а slight decreаse in 2021 to 37.4 %, which wаs most likely due to the climаtic conditions of this period. Of the two selected drugs with seemingly the sаme spectrum of аction, the best results were obtаined on the deer of the second experimentаl group, where the drug "Dektomаx" wаs used, while the EI wаs significаntly (two times) lower thаn in the first experimentаl group аnd аmounted to 16.7 % versus 33. 3 %, аnd АI - 0.03, аgаinst 0.83.