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Search results: Ye.B. Nikitin

Number of results: 17


Heterological serological diagnostics of nodular cattle dermatitis
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he article is devoted to the current problem of laboratory diagnosis of nodular dermatitis in cattle. Specific tools and methods for diagnosing t his disease have not yet been developed. There is evidence of an antigenic relationship between the causative agent of nodular dermatitis of cattle and the causative agent of sheep pox. The possibility of using means and methods of serological diagnosis of sheep pox for the diagnosis of ND cattle is being considered. Reducing the timing of diagnosis helps to increase the effectiveness of ongoing therapeutic and antiepizootic measures.

Year of release: 2020
Number of the journal: 1(77)

Development of agricultural biotechnology in Kazakhstan
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In the article the main issues and trends of agricultural biotechnology development in Kazakhstan, prospects of biotechnology application in the production of agro-industrial complex of the country have been considered. Data has been presented on: what researches are being carried out by scientific organizations in the field of agricultural biotechnology with attraction of leading foreign scientists and scientific organizations; what recommendations for technologies have been developed and what new biotechnological products have been created in all branches. Promising technologies in the field of biotechnologies in AIC for Kazakhstan as well as keys to the success in the development of science and technologies in AIC have been indicated.

Year of release: 2015
Number of the journal: 3(59)

Application of the "Step by Step" technology in the teaching of younger schoolchildren as a means of improving the quality of education
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The renewal of the paradigm of 12-year education is associated with the formation of key competencies in students that help to learn, understand and develop the world around. The developed set of tasks is designed to develop the need to transform educational material with the aim of improving the quality of education. This material is compiled in accordance with the age characteristics of primary school students, is aimed at organizing the processes of mastering new ways of acting, which will contribute to the formation of educational and cognitive activities. The issues discussed in the article will allow us to implement a competence approach in the education of students. The tasks presented in the article are intended to activate the educational motivation, the organization of independent cognitive activity, contribute to the systematization and objectification of the processes of monitoring and evaluation of educational activities.

Year of release: 2016
Number of the journal: 3(63)

To the question of the treatment and prevention of intestinal diseases by bacteriophages
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The proposed article presents theoretical studies of the treatment and prevention of intestinal infections of farm animals and birds. The relevance of the topic is due to the emergence and spread of infections resistant to antibiotics associated with the widespread use of antibiotics in clinical medicine, veterinary medicine and agriculture, which reduces the effectiveness of antibiotics in the fight against intestinal infectious diseases and their treatment

Year of release: 2018
Number of the journal: 3(71)

Development of technology for the purification of water from mercury pollution
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The object of the study was water and bottom sediments of the lake Balkyldak of Pavlodar region, contaminated by mercury wastes.The purpose of the work is the creation of innovative cost-effective technologies for deep cleaning of aqueous media from mercury to the level of 1-10 parts per trillion, which will be tested for the cleaning of the lake Bylkyldak facilities in Pavlodar region.

Year of release: 2019
Number of the journal: 4(76)

Studying the possibility of using tools and diagnostic methods for adenovirus infection of cattle in infectious hepatitis dogs
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The article is devoted to the current problem of differential diagnosis of diseases of viral etiology in small domestic animals. Viral diseases of dogs and cats are widespread among both high-breed and outbred animals. The spread of diseases is facilitated by an increase in the number of small domestic animals, the popularization of pet keeping, and cross-border operations related to the movement of animals. The possibility of using means and methods of diagnosing adenovirus infection in cattle, tentative diagnosis and differentiation of infectious hepatitis in dogs from plague of carnivores and parvovirus enteritis is being considered. Reducing the timing of diagnosis helps to increase the effectiveness of ongoing therapeutic and antiepizootic measures. The analysis of the place of carnivorous infectious hepatitis in the structure of the incidence of dogs is given. The article describes the research in the field of the possibility of using the established antigenic relationship of representatives of the adenovirus family for the in vivo and posthumous diagnosis of infectious diseases of small pets. The authors proposed a method for performing the production of serological reactions for the diagnosis of infectious hepatitis in dogs using diagnostic tools for adenovirus infection in cattle.

Year of release: 2019
Number of the journal: 3(75)

The study of nutritional values and organoleptic properties of poly-grain extruded mixture
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Among many environmental conditions that affect a person, the most important factor is nutrition. Today, there is no doubt that there is a direct link between nutrition, health and disease. Proper nutrition ensures normal growth and development of a person, contributes to the prevention of diseases, has a positive impact on life expectancy and creates conditions for adaptation to the environment. A very actual topic for public catering in the Republic of Kazakhstan, namely for fast food companies, is the development of new recipes and culinary products from relatively inexpensive vegetable raw materials, as well as qualitatively new food products with a purposefully changed chemical composition. One of the main ways of solving problems of expanding the production of products for quick service, as well as products for dietary and therapeutic and preventive nutrition is the use of sprouted grains and beans. In this article, poly-grain mixtures of sprouted wheat and extruded soy beans in different ratios are studied and considered. Poly-grain mixtures developed by us are balanced in nutrients, vitamins and amino acids. The research was carried out on the basis of the accredited testing laboratory of RUBICOM enterprise LLP and the scientific laboratory of the Innovative Eurasian University. The purpose of this work is to study a promising method for increasing the nutritional value of grain mixtures by extruding them at different temperature conditions. To achieve this goal, the following tasks were planned: – to study the grain of soy beans and sprouted wheat on the organolepti c characteristics and chemical composition of the poly-grain mixture; – examine the chemical composition of the poly-grain mixture before extrusion; – choose the optimal mode of the extrusion process; – study and analyze the chemical composition of poly-grain extruded mixture; It should be noted that we have developed for the first time the optimal technological mode of extrusion of poly-grain mixture from sprouted wheat and soy beans, and the physical and chemical composition of the poly-grain mixture was studied. The results of the research presented in this paper are the basis for the development of recipes and technologies for fast food products. The reliability and validity of scientific statements is confirmed by the choice of modern methods of chemical composition analysis.

Year of release: 2020
Number of the journal: 2(78)

The detection of the virus of infectious rhinotracheitis in cattle by polymerase chain reaction
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The article is devoted to the current problem of differential diagnosis of diseases of viral etiology in farm animals. Viral diseases are currently widespread, occupy a leading role in the infectious pathology of farm animals, causing enormous economic damage. Given the magnitude of animal vaccine prophylaxis, in order to increase the effectiveness of antiepizootic measures, the urgent issue i s the development of methods for the rapid and effective detection and differentiation of field and vaccine strains of the infectious rhinotracheitis virus in cattle. The possibility of using a polymerase chain reaction to identify and differentiate a vacc ine strain from epizootic strains and isolators of the cattle infectious rhinotracheitis virus is considered. In the process of research, a PCR-RFLP analysis method was developed to detect the IRT virus in the test material. The PCRRFLP analysis method was used to identify and differentiate the vaccine strain TK-A form epizootic strains and isolators of the cattle IRT virus. The principle of PCR, based on repeated repetition of DNA cycles, annealing and synthesis, which leads to an increase in the number of specific DNA fragments of the pathogen, allows you to take into account the results of PCR in an agarose gel. Analysis time is about 30 hours. The sensitivity of detecting viral DNA is 1-10 picograms (102 TCD). Due to characteristics such as relative simplicity and reaction rate, high sensitivity, specificity and reproducibility, PCR has recently become widespread in basic and applied research in various fields of biological science, including veterinary virology. The results obtained during the studies show that the use of PCR-RFLP allows to differentiate field and vaccine strains and isolates of the IRT virus with a high degree of reliability. The use of PCR -RFLP analysis increases the efficiency and informativeness of studies in the molecular epizootol ogy of cattle RTI, as it allows not only to identify the DNA of different virus strains regardless of their nature, but also to differentiate between them, including differentiating the strain TK-A used for the production of attenuated vaccines against epizootic strains and isolates of the virus.

Year of release: 2020
Number of the journal: 2(78)

Study of the possibility of using heterologous preparations in the serologic diagnosis of carnivorous plague
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Main problem: The genus Morbillivirus of the family Paramyxoviridae includes agents of morbilli, plague of cattle, carnivores and small ruminants. Plague agent of carnivores causes a dangerous disease of fur animals and dogs, which in many cases ends in death. The disease is widespread everywhere, including Kazakhstan. In our country, the mixed form of the disease is most common, and the least common is the skin and nervous forms. The chronic course of plague is observed to a greater extent with the nervous form, subacute - with mixed and intestinal, acute - with cutaneous and pulmonary forms of the disease. Goal: Veterinarians have significant difficulties in diagnosing carnivorous plague. Despite the fact that serological methods for diagnosing this infection have been developed and applied for research purposes, the lack of commercial diagnostic preparations on sale significantly restrains their use by practical veterinary workers. Therefore, the diagnosis of plague of carnivores is mainly established on the basis of the symptom complex of the disease and the data of epizootic and pathological anatomical studies, the results of which are largely similar to those in some other diseases of carnivores. Scientific research in the field of means and methods of laboratory diagnostics of carnivore plague, applicable in practical veterinary medicine, is in great demand. Methods: Considering the fact that the carnivores plague and cattle plague are antigenically closely related, studies were carried out to determine the possibility of using means and methods for diagnosing cattle plague for serological diagnosis of carnivores plague. Results and their significance: It has been established that the use of means and methods for diagnosing cattle plague makes it possible to diagnose carnivore plague at all stages of the course of the disease, their use makes it possible to reliably establish a diagnosis of the disease in fur-bearing animals and dogs in a short time after the receipt of samples of material for research, even in cases of mixed viral and bacterial infections; and also to differentiate the plague of carnivores from diseases similar to it - parvovirus enteritis, infectious hepatitis and a number of others.

Year of release: 2020
Number of the journal: 3(79)

Current state and prospects of fortified food production in Russia and Kazakhstan
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Main problem: The article examines promising directions in the production of fortified food products in Kazakhstan and Russia. The authors carried out an analytical review of the current state of production of fortified bakery products on the territory of the Russian Federation and the Republic of Kazakhstan. Anemia has been described as one of the most common consequences of micronutrient deficiencies. The problems associated with anemia lead to serious complications for the health and well-being of people. Lack of folic acid and vitamins in the diet is the cause of cardiovascular disease, maternal and child mortality. Purpose: The article considers the problem of significant deviations in nutrition of almost all groups of the population of Russia and Kazakhstan, regardless of lifestyle, habits or income level, which have an extremely negative effect on the health of the nation: the average life expectancy decreases, the productivity of the working-age population and resistance to diseases decrease. Methods: Based on the studied domestic and foreign technical literature, patent information, an analytical review of the current state and prospects of the production of fortified food products in the Russian Federation and the Republic of Kazakhstan was carried out. Results and their significance: The authors have shown that food fortification is the practice of purposefully increasing the content of vitamins and microelements in food to improve the nutritional quality of food and have a positive effect on human health. The authors considered the question of how the problem of micronutrient deficiency, including iron, is currently being effectively solved in many countries by enriching food products of mass consumption with vitamins and minerals.

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Development of a technology for producing organic fertilizers based on catalytic processes
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The article is devoted to the development of a technology for obtaining organic fertilizers from poultry waste based on biocatalytic processes. Currently, many poultry farms have become sources of environmental pollution, thereby causing serious environmental problems and economic and social damage. The problem of reliable protection of the natural environment from pollution by bird droppings is currently relevant. In the area of operation of large poultry farms, air pollution by microorganisms, dust, foul-smelling organic compounds, which are decomposition products of organic waste, as well as nitrogen, sulfur, and carbon oxides, is possible. Bird droppings contain acids, nitrogen, phosphorus and potassium, heavy metals. The content of nitrogen, phosphorus and potassium changes dramatically depending on the quantity and quality of the feed. Humic substances isolated from brown coal have a sufficiently high sorption activity and are used as cheap sorbents for solving a number of environmental problems in industry. These studies are aimed at minimizing the main disadvantage of the anaerobic digestion process, the low reaction rate, which leads to the need to create large-volume bioreactors. On the basis of the research carried out, a technology has been developed for obtaining organic fertilizers from poultry waste by the method of anaerobic fermentation of chicken manure with humidity at a temperature of 27 °C - 50 °C with the addition of sodium humate. The resulting fertilizer is intended for use in agricultural production, horticulture, floriculture, forestry, municipalities, in household plots in order to increase the yield and quality of crop production. For the developed technology, an application has been submitted for obtaining a Patent for the invention of the Republic of Kazakhstan «Method for producing organic fertilizers» (priority No. 2021-22818, dated July 13, 2021). The invention allows to significantly reduce the time of fermentation, to enrich the product with organic and mineral products contained in sodium humate, to convert the salts of heavy metals into an insoluble state, to improve the environmental friendliness of the method.

Year of release: 2021
Number of the journal: 4(84)

Results of application of organomineral fertilizer obtained with biocatalytic processes
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Main problem: Bird droppings and animal manure contain acids, nitrogen, phosphorus and potassium, heavy metals. The content of nitrogen, phosphorus and potassium changes dramatically depending on the quantity and quality of the feed. Purpose: To study the effectiveness of the use of organomineral fertilizer from poultry waste, obtained using biocatalytic processes on the growth and development of plants. Methods: An application for a Patent for the invention of the Republic of Kazakhstan "Method of obtaining organic fertilizer" has been submitted for the developed technology for obtaining organic-mineral fertilizer. In the soil intended for growing seedlings of tomatoes of the “Pepper-shaped Orange” variety and peppers of the “Bogatyr” variety, the resulting fertilizer was applied in the amount of 1 kg per 1 m2 of soil, which was dug to a depth of 8-10 cm and used to grow seedlings in closed ground and subsequent for planting it in open ground. Soil without fertilization was used as a control. The process of soil preparation for open ground, intended for planting seedlings, was carried out for the experimental field with the introduction and control without fertilization. Experimental and control studies were carried out under equivalent climatic conditions, the scheme and technology of watering plants. In total, 100 bushes of each plant species were used in the experiment. Results and their significance: The use of the obtained organomineral fertilizer allows to increase the yield of vegetable crops (tomatoes, peppers) by 20-25% compared to the control. In addition, in the experimental samples, an increase in the number of fruits on a bush was noted with an increase in their size, a decrease in the growing season and the number of damaged fruits.

Year of release: 2022
Number of the journal: 2(86)

Improving the means and methods for the prevention and treatment of postpartum paresis of cows
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In modern conditions, the problem of the incidence of cows with high productivity in the postpartum period is relevant. It is these animals that are more susceptible to various diseases, primarily due to violations of the technology of keeping and feeding, weak body resistance, heavy loads during fruiting and milk production. It is known that as a result of various metabolic disorders, calving of cows in farms proceeds with various deviations. The lack of calcium in the blood due to hormonal disorders leads to hypocalcemia and paresis after calving, which is often the cause of death of animals. Therefore, the improvement of prevention schemes and treatment of puerperal paresis in cows is very relevant. Purpose of the article – Improvement of preventive and therapeutic measures for postpartum paresis in cows on the basis of the Tassu farm in Akmola district. The work was carried out under production conditions on a livestock farm in the Akmola region, in the farm "Tassu" in the spring and summer. The object of the study were black-motley cows with a pregnancy period of 250-260 days. 22 animals were selected in the experimental group, 12 animals in the control group. The average weight of animals is 567 ± 34.5 kg, age 5-6 years. The cows of the experimental group received injections of the drug Karsulen, which included: 2 % Cardus marianus D3, 1 % Sulfur D6 and 1 % Arsenycum album D6. Karsulen was administered to cows intramuscularly or subcutaneously at the rate of 1 ml per 100 kg of animal weight, the dose volume should not be less than 5 ml per animal. In the postpartum period, in the complex therapy of postpartum paresis in cows, Karsulen is used intramuscularly or subcutaneously 1-2 times a day. The use of the drug Karsulen with the composition: 1 % Cardus marianus D3, 1 % Sulfur D6 and 1 % Arsenycum album D6 has a regulatory effect on protein, carbohydrate, fat metabolism, restores impaired function of the gastrointestinal tract, liver and kidneys.

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Analysis of the epizootic situation of canine distemper among dogs in the city of Tula
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Main problem: At present, service, decorative and hunting dog breeding is intensively developing in Russia and in the Tula region. So, in 2020, 119 high-breed puppies were registered in the Book of Pedigree Breeding, and in 2021 already 164 puppies, mostly decorative and service breeds. Thus, the number of pedigreed dogs susceptible to various infectious diseases is increasing annually. Canine distemper is one of the most widespread viral diseases of dogs all over the world, including in Russia. Analyzing the "Patient Admission Logs" of veterinary clinics in the city of Tula, it was found that 44 % of dogs that fell ill with diseases of infectious etiology were diagnosed with canine distemper. Purpose: The aim of our research was to study the epizootological features of the course of canine distemper among dogs in the Tula region of Russia. To achieve the intended goal, it was necessary to solve the following problem: to analyze the breed and age factors for the incidence of canine distemper. Methods: Analysis of data from private veterinary clinics reporting on the incidence of small domestic animals was used. Results and their significance: As a result of studying the epizootic situation of canine distemper among dogs, the following data were obtained: The disease is observed throughout the year and has the character of undamped fluctuations subject to sharp seasonal changes. The maximum number of diseased dogs in all years of observation is recorded in March - 6.2 cases per 1000 individuals. It has been established that among the infectious diseases of dogs, distemper occupies the first place, and there is an annual increase in the number of dogs with distemper.

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Technology for the preparation of a fermented milk product with the addition of watermelon juice
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Main problem: the development of a new direction in the food industry – the so-called functional nutrition, which means the use of such products of natural origin, which, when systematically used, have a regulatory effect on the body as a whole or on its specific systems and organs, has been widely recognized all over the world: immunostimulants, biocorrectors for blood pressure, cholesterol levels, etc. When creating functional dairy products, ingredients of plant origin are increasingly included in recipes, which are sources of dietary fiber, vitamins and microelements, organic acids and other biologically active compounds, which can also serve as flavor fillers. Purpose: to develop a technology for the preparation of a fermented milk product with the addition of an extract from gourds. To do this, it was necessary: to select the amount (dose) of the yoghurt starter introduced into the prepared milk, and also to investigate the effect of temperature on the fermentation process of the drink; to investigate the amount (dose) of the vegetable filler introduced into the mixture and its effect on the organoleptic characteristics of the drink; to investigate the organoleptic properties of the drink with the melon culture extract added to it before fermentation and after fermentation; to investigate the effect of vegetable filler on the fermentation process; to investigate the effect of the dose of vegetable filler on the process of acid formation; conduct microbiological studies of milk and vegetable yogurt; to investigate the change in the acidity of milk and vegetable yogurt during storage; to investigate the effect of the freezing process on the chemical composition and biological value of the extract. Methods: when performing the practical part of the work, physicochemical, organoleptic and microbiological studies were used. Results and their significance: in the course of the study, it was concluded that by adding an extract of gourds to the technology for the preparation of fermented milk products, a new fermented milk product can be obtained that meets all physicochemical, organoleptic and microbiological requirements. As a result, an original method for the production of a fermented milk drink containing an extract from a melon culture was developed.

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An innovative approach to the development of biotechnology of specialized products for the nutrition of athletes
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Main problem: the article substantiates the relevance of the development and production of specialized food products for athletes and the population involved in sports, fitness as part of a healthy lifestyle. Purpose: development of specialized food products with a general health-improving character, intended for the population involved in sports, fitness and experiencing increased physical exertion. Methods: the obtained results were statistically processed using the methods of correlation and regression analysis and standard software packages "Statistica 6.0". Measurements of chemical, microbiological, organoleptic parameters were carried out using standard methods and modern instruments: an analyzer, an analytical analyzer electrophoresis cell, a high-performance liquid chromatograph, and others. Results and their significance: a scientific concept for the creation of fermented specialized milk-based food products for sports nutrition was formulated; biotechnologies for specialized food products for athletes were developed. For the practical implementation of the results of analytical and experimental studies, packages of regulatory and technical documentation for the production of new products have been developed, which have been tested in the production conditions of existing enterprises in the dairy industry.

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