he article presents the scientific rationale for a promising trend - a product with a high protein content, enriched with functional ingredients for specialized (herodietic) nutrition. Based on the results of our own research, we determined the component composition of the fermented product and the main dairy raw material - buttermilk, characterized by high biological value. The new product contains an antioxidant complex and a herbal ingredient that expands the product range.

In the article the main issues and trends of agricultural biotechnology development in Kazakhstan, prospects of biotechnology application in the production of agro-industrial complex of the country have been considered. Data has been presented on: what researches are being carried out by scientific organizations in the field of agricultural biotechnology with attraction of leading foreign scientists and scientific organizations; what recommendations for technologies have been developed and what new biotechnological products have been created in all branches. Promising technologies in the field of biotechnologies in AIC for Kazakhstan as well as keys to the success in the development of science and technologies in AIC have been indicated.

Gellants are food additives used to increase the dencity and create a certain structure of the food product. In the dairy industry, there is an active work at developing new technologies for fermented milk products, which allows improving organoleptic quality indicators with the use of gellants. In this article, an analysis of the assortment of gellants used in the dairy industry was analyzed.

The article emphasizes the importance of carrying out breeding work with Kazakh horses of the zhabe type, which are of great importance for the productive horse breeding industry. The results of selection experiments on improving breeding and productive qualities are presented on the example of the Kazakh zhabe horses of Seletiinsky factory type at a stud farm "Altai Karpik Saydaly-Sartoka" of the Irtysh district of the Pavlodar region.

The article is devoted to the actual problem of tooth loss and solving the problem through the development of tissue engineering. Tissue engineering is a set of methods and procedures aimed at the regeneration of biological tissues. It includes a triad of basic elements: stem cells, extracellular matrix or scaffold, growth factors and signaling pathways. An important role in solving the existing problems is associated with stem cells. Dental stem cells are capable of self-renewal and differentiation. Based on the analysis, it was found that each type of dental stem cells has its own characteristics and applications not only in dentistry, but also in other areas of medicine.

The work is intended to determine the etiopathogenetic principles of animal prevention in gynecological pathology and issue a theoretical justification. At the same time, improving preventive measures aimed at preserving their health, increasing the productivity of animals in modern conditions of animal husbandry and ensuring high productivity of cows. In this regard, the main task is to develop ways to increase insemination of cows with the use of complex and homeopathic treatment. For the first time in the East Kazakhstan region, new scientific data on the main etiological factors leading to gynecological pathology and infertility of cows were obtained. Work has been carried out to improve measures aimed at preventing infertility of cows in the conditions of dairy farming in this region and stimulating increased insemination. New schemes for stimulating increased insemination using hormonal, homeopathic and other drugs have been tested. As a result, an increase in the productivity of cows was revealed, and stimulation schemes were introduced. For the first time in the farm" kamyshinskoye" an economic assessment of the damage from infertility of cows is given. Research work is done between 2016 and 2019 years by the Department of veterinary medicine of the State University named after Shakarim, in the laboratory "Agrotechnopark" of the State University named after Shakarim and national University of veterinary medicine and biotechnology named after Lviv and in the farm "kamyshinskoye" of the Shemonaikha district of East Kazakhstan region. To enhance the insemination of cows 3 groups of animals was obtained: animals of group I were not subjected to insemination; animals of group II for 1-3 hours before insemination grafted surfagon 3 ml (15 mg), 15 ml of Catosal, 15 ml habilita-Se, 10 ml uteroton; 8 days prior to insemination progesterone 2,5% 2 ml, Catosal 15 ml, habilita-Se 15 ml; cows of group III for 30-60 minutes before insemination were given Ovariovitis, and after fertilization Ovariovitis for 25-30 days and liarcine 5 ml. According to the results of drawings based on complex and homeopathic preparations, low costs were shown.

Main problem: The genus Morbillivirus of the family Paramyxoviridae includes agents of morbilli, plague of cattle, carnivores and small ruminants. Plague agent of carnivores causes a dangerous disease of fur animals and dogs, which in many cases ends in death. The disease is widespread everywhere, including Kazakhstan. In our country, the mixed form of the disease is most common, and the least common is the skin and nervous forms. The chronic course of plague is observed to a greater extent with the nervous form, subacute - with mixed and intestinal, acute - with cutaneous and pulmonary forms of the disease. Goal: Veterinarians have significant difficulties in diagnosing carnivorous plague. Despite the fact that serological methods for diagnosing this infection have been developed and applied for research purposes, the lack of commercial diagnostic preparations on sale significantly restrains their use by practical veterinary workers. Therefore, the diagnosis of plague of carnivores is mainly established on the basis of the symptom complex of the disease and the data of epizootic and pathological anatomical studies, the results of which are largely similar to those in some other diseases of carnivores. Scientific research in the field of means and methods of laboratory diagnostics of carnivore plague, applicable in practical veterinary medicine, is in great demand. Methods: Considering the fact that the carnivores plague and cattle plague are antigenically closely related, studies were carried out to determine the possibility of using means and methods for diagnosing cattle plague for serological diagnosis of carnivores plague. Results and their significance: It has been established that the use of means and methods for diagnosing cattle plague makes it possible to diagnose carnivore plague at all stages of the course of the disease, their use makes it possible to reliably establish a diagnosis of the disease in fur-bearing animals and dogs in a short time after the receipt of samples of material for research, even in cases of mixed viral and bacterial infections; and also to differentiate the plague of carnivores from diseases similar to it - parvovirus enteritis, infectious hepatitis and a number of others.

In the last decades of the twentieth century, in the national economy of many countries, organochlorine pesticides were most widely used, characterized by stability in the external environment, the ability to cumulate in various tissues of organisms. Lindane (the gamma isomer of hexachlorocyclohexane) is listed as a restricted persistent organic pollutant and is an ecotoxic substance with severe and chronic effects on the human body. The study of the effect of lindane on carbohydrate metabolism at the present stage is still insufficient. This fact led to the study of the effect of γ-HCH on the insulinogenic function of the pancreas in in vivo and in vitro experiments. In experiments in vivo, the animals of the experimental groups were once orally administered γ-HCH at a dose equal to 1/5 DL50. Isolated pancreatic islets, precipitated in vitro and fixed on mica plates, were exposed to γ-HCH in amounts equivalent to 1/5 to 1/4 DL50. Paraffin sections of pancreatic tissue from experimental and control animals were stained with aldehyde fuchsin according to Gomori, and tissue preparations were also examined by a highly specific method for detecting insulin in β-cells using diethylpseudoisocyanin staining, followed by examination of the preparations in the ultraviolet light of a luminescent microscope.The same methods were used to study preparations of isolated pancreatic islet tissue on the 4th day of cultivation. The influence of orally administered γ-HCH on the level of immunoreactive insulin in the blood of experimental animals was also studied. The insulin level was determined by the enzymatic-immunological method. The concentration of IRI was established before the start of the experiment and 4-4.5 hours after acute inoculation. Results and their significance. In the study of stained preparations of the pancreas of experimental animals, numerous islets of ordinary sizes were revealed, the cytoplasm of which was filled with aldehyde-fuccin granularity in quantities indistinguishable from those observed by microscopy of preparations of control animals. The value of the fluorescence coefficient in the histofluorimetric study of the control and experimental preparations did not differ significantly. However, the content of IRI in the blood serum showed a distinct decrease in the first hours after priming. In experiments in vitro, when studying the effect of γ-HCH on cultured tissue, introduced into the nutrient medium on the second day, in the field of view of the microscope, single, small pancreatic islets were revealed. Their number on a constant area of the plates was significantly lower than the value of the same indicator in the study of control preparations. Thus it has been shown that γ-HCH does not affect the histostructure of the endocrine pancreas, but causes a significant decrease in IRI in the blood serum, as well as a change in the histochemical characteristics of cultured β-cells.

The sharp deterioration of the environmental situation in the Republic of Kazakhstan, associated with human activity and the impact of harmful factors on the environment, affected the quality of food consumed by insufficient consumption of domestic fruit and vegetable products containing vitamins, minerals of an alkaline nature, carbohydrates, vegetable proteins, pectin substances and active fiber. The problem of improving the quality of growing and processing fruits and vegetables is one of the main tasks of the food independence of the Republic of Kazakhstan. Currently, the share of large vegetable farms in the Republic of Kazakhstan is only 10%. The volume of industrial processing of fruit and vegetable crops in Kazakhstan is insignificant. The demand for domestic fruit and vegetable products in the country is met only by 35-40%, most of it is imported from countries such as Russia, Kyrgyzstan, Uzbekistan and China. There is a need for the development of organic vegetable growing in Kazakhstan and the creation of new types of domestic fruit and vegetable products containing a sufficient amount of useful and active substances that can bind and remove toxic substances and radioactive elements from the human body. The purpose of the work is to improve the technology of fruit and vegetable semi-finished products with natural spicy and aromatic additives, to develop a multi-component recipe for vegetable mixtures and a method for producing quick-frozen vegetable mixtures with high quality indicators. These studies were aimed at studying the chemical composition and functional and technological properties of vegetables and spicy - aromatic plants for the content of biogenic compounds: carbohydrates, proteins, vitamins, essential oils, minerals and other substances. The structure of plant tissues was studied and the mass fraction of moisture, dry matter, fiber, mono - and disaccharides, pectin substances, vitamin C was determined, the formulation and production technology of Assorted vegetable mixtures were developed, and quality indicators were determined.

This article discusses the current data on the biochemical properties of bacteria of the genus Lactobacillus and their use in industry, particularly in dairy production and biotechnology. Microorganisms of the genus Lactobacillus have antagonistic activity against pathogenic microorganisms and perform an immunomodulatory function. The positive effect of lactobacilli on human health explains their active use in probiotics. The positive effects of normal intestinal microflora and probiotics are mainly due to bifidobacteria and lactobacilli. In probiotic therapy, various types of bacteria of the genus Lactobacillus are used, such as: L. acidophilus, L. rhamnosus, L. plantarum, L. fermentum, L. delbrueckii subsp. bulgaricus, L. casei, L. paracasei. In recent years, the biotechnology of probiotics has been intensively developing - drugs used for the correction and prevention of microecological disorders in the gastrointestinal tract of humans and animals. An urgent issue is obtaining new data on the biological properties of lactobacilli, creating new probiotic preparations based on them using modified approaches to cultivation. The results of determining the species L. acidophilus L. casei, L. rhamnosus, L. paracasei, obtained by the classical biochemical identification method based on saccharolytic activity, complicating species identification, are comparable to the molecular genetic method. In the case of L. casei, L. rhamnosus, L. paracasei, it is necessary to carry out modern identification methods based on the polymerase chain reaction, since their biochemical properties are similar, which makes it difficult to carry out species identification. The molecular genetic method is a valuable addition to the intergeneric and species identification of lactobacilli, given the variability of the classical biochemical method.

The production of vaccine preparations before release requires standardization of their immunobiological parameters, especially safety and immunogenic efficacy. An indicator of the immunogenic effectiveness of the lumpy skin disease vaccine is the resistance of vaccinated cattle against the virulent virus. However, according to preliminary studies, the virulent control virus did not always cause clinical disease with characteristic symptoms when infected subcutaneously. The purpose is to develop a biological model in the form of a complex consisting of a pathogenic virus, a method of infection and a susceptible animal to assess the immunogenicity of a lumpy skin disease vaccine. Local cattle, intact from lumpy dermatitis, were used to reproduce lumpy dermatitis and develop the causative agent of the disease. As the initial infectious virus, we used a 20 % tissue suspension of nodules (skin nodules) obtained from cattle that fell ill with lumpy dermatitis in the field in the Atyrau region in 2016. As a viral mass to control immunogenicity, a 20 % suspension of skin nodules and edematous skin tissue at the site of the pathogen injection, obtained after the “refreshment” of the virus in animals, were used. The disease was reproduced by infection with the test suspension of the virus intradermally, subcutaneously, intravenously at a dose of 0.5 cm3 and titration on the skin of the animal. The effectiveness of the biological model was assessed by morbidity, severity of the course and severity of the manifestation of the disease. During primary intradermal infection with a field isolate of the virus, the disease manifested itself in one of three animals in the form of hyperthermia, depression, lacrimation, and the appearance of several nodular nodules in the skin of animals. The refreshed tissue virus caused clinical disease both in subcutaneous, intradermal and intravenous infection. But the clinical signs of the disease were more pronounced with intradermal inoculation of the virus, and with intravenous inoculation, it manifested itself in a more severe form with a fatal outcome. Inoculation of the virus intradermally into different areas of the skin led to the development of an independent skin lesion in each infected point in the form of painful edema, followed by necrosis, the size and intensity of which depended on the dose of the injected virus. This development of skin pathology made it possible to work out a method for determining the virus titer in vivo. The tissue virus obtained from the edematous tissue at the site of the pathogen injection was guaranteed to cause clinical disease in cattle during intradermal inoculation and made it possible to evaluate the immunogenic efficacy of the produced batches of vaccine against lumpy dermatitis.

Main problem: the article substantiates the relevance of the development and production of specialized food products for athletes and the population involved in sports, fitness as part of a healthy lifestyle. Purpose: development of specialized food products with a general health-improving character, intended for the population involved in sports, fitness and experiencing increased physical exertion. Methods: the obtained results were statistically processed using the methods of correlation and regression analysis and standard software packages "Statistica 6.0". Measurements of chemical, microbiological, organoleptic parameters were carried out using standard methods and modern instruments: an analyzer, an analytical analyzer electrophoresis cell, a high-performance liquid chromatograph, and others. Results and their significance: a scientific concept for the creation of fermented specialized milk-based food products for sports nutrition was formulated; biotechnologies for specialized food products for athletes were developed. For the practical implementation of the results of analytical and experimental studies, packages of regulatory and technical documentation for the production of new products have been developed, which have been tested in the production conditions of existing enterprises in the dairy industry.

The article is devoted to the development of technology for meat products of the "Halal" category. The authors consider the main problems of the meat industry in the field of the development of meat products of the "Halal" category in accordance with the National Program "Development of meat farming for 2018-2027", as well as the peculiarities of the production of meat products of the "Halal" category in Kazakhstan. A technology for the production of sausages for frying of the "Halal" category based on minced turkey and beef meat for functional nutrition has been developed, organoleptic, physico-chemical parameters of the new product have been studied. The mass fraction of protein is 8 %, the mass fraction of fat is 7 %. The authors of the article have developed a technological scheme for the production of a new product of the "Halal" category, which includes: acceptance of raw materials, cutting, deboning, veining, grinding to 2-3 mm of meat raw materials. At the next stage, meat is cooked at t 2-4 0C for 12 hours, minced meat is prepared (adding jerusalem artichoke 3 %, adding vitamin premix "Rovifarin" in an amount of 1.0 %, as well as spices and garlic according to the developed recipe). At the next stage, the minced meat is stuffed into natural shells with a diameter of (32-44) mm, cooled at t (4 + 2) 0C, stored at t (4 + 2) 0C for 36 hours. During the microstructural study of a sample of boiled and smoked sausage produced using traditional technology, it was found that the mass of the sample was formed from large fragments of muscle, fat and connective tissue (0.7-1.4) microns. The layout of the structural elements of the minced meat is dense, vacuoles and microcapillaries are rounded in shape, sometimes without clear boundaries, merged, with a size of (60-300) microns.